To shed some light on titer testing, the following is from Page 19 the American Animal Hospital Association's 2003 Canine Vaccine Guidelines under the heading "Serological Tests to Monitor Immunity: it states that "Although the committee does not feel it is necessary to determine titers to these core viruses on an annual basis because of the long minimum DOI [duration of immunity] for these products, titers can be used for your and/or your client's assurance that the animal has immunity. Experience with postvaccination titers for CDV [distemper], CAV [hepatitis], and CPV [parvo] shows that sterile immunity lasts for years…........The primary reason for the test is to ensure that you have a positive test after completing the puppy vaccination series."

    From Page 16 of the 2003 AAHA Guidelines it reports that, "The MLV [modified live virus (such as distemper, hepatitis, parvo)] vaccines create an immunity that is similar to immunity after an animal recovers from infection." Further, on Page 17, " When MLV [modified live virus (such as distemper, hepatitis, parvo)] vaccines are used to immunize a dog, memory cells develop and likely persist for the life of the animal."

    Dr. Alice Wolf, Professor of Small Animal Internal Medicine at Texas A&M College of Veterinary Medicine, stated in an address (Vaccines of the Present and Future http://www.vin.com/VINDBPub/SearchPB/Proceedings/PR05000/PR00141.htm) at the 2001 World Small Animal Veterinary Association World Congress that: "MLV vaccines generally produce longer duration, more solid immunity. Both humoral and cell-mediated immune systems participate strongly in the response. A single vaccination may provide significant immunologic memory (as long as there is no maternal AB interference). MLV vaccines do not, or only infrequently, require revaccination …"..

    This is what Dr. Ronald Schultz had to say in his 2007 presentation to the AKC Canine Health Foundation entitled, What Everyone Needs to Know About Canine Vaccines and Vaccination Programs:

    "An antibody titer no matter how low shows the animal has immunologic memory since memory effector B cells must be present to produce that antibody. Some dogs without antibody are protected from disease because they have T cell memory, that will provide cell mediated immunity (CMI). CMI will not protect from reinfection, but it will prevent disease."

    "My own dogs, those of my children and grandchildren are vaccinated with MLV CDV, CPV-2, CPI, andCAV-2 vaccines once as puppies after the age of 12 weeks. An antibody titer is performedtwo or more weeks later and if found positive our dogs are never again vaccinated. " " he further elaborates: "I have used this vaccination program with modifications (CAV-2 replaced CAV-1 vaccines in 1970's and CPV-2 vaccines were first used in 1980) since 1974! I have never had one of our dogs develop CDV, CAV-1 or CPV-2 even though they have had exposure to many dogs, wildlife and to virulent CPV-2 virus. You may say that I have been lucky, but it is not luck that protects my dogs, it is immunologic memory."

    The vaccines in the quote above are CDV (distemper), CPV-2 (parvovirus), CPI (canine parainfluenza), and CAV-2 (hepatitis), and Dr. Ronald Schultz is the Chair of the Department of Pathobiological Sciences at the University of Wisconsin School of Veterinary Medicine. His challenge and serological studies on canine vaccines form a large part of the scientific data base upon which the 2003 and 2006 American Animal Hospital Association's Canine Vaccine Guidelines are based, as well as the 2007 World Small Animal Veterinary Association's Vaccine Guidelines.

    Combination Vaccines, Multiple Shots–on Page 16 of the 2003 AAHA Guidelines under Immunological Factors Determining Vaccine Safety, it states that: "Although increasing the number of components in a vaccine may be more convenient for the practitioner or owner, the likelihood for adverse effects may increase. Also, interference can occur among the components. Care must be taken not to administer a product containing too many vaccines simultaneously if adverse events are to be avoided and optimal immune responses are sought. "

    Duration of Immunity: The Rabies Vaccine Challenge - Show #185 Animal Talk Radio Show 7/30/08 http://www.blogtalkradio.com/animaltalknaturally/2008/07/30/Duration-of-Immunity-The-Rabies-Vaccine-Challenge-Show-186

    Duration of Immunity to Canine Vaccines: What We Know and Don't Know, Dr. Ronald Schultz http://www.cedarbayvet.com/duration_of_immunity.htm

    What Everyone Needs to Know about Canine Vaccines, Dr. Ronald Schultz

    Vaccination: An Overview Dr. Melissa Kennedy, DVM360 http://veterinarycalendar.dvm360.com/avhc/article/articleDetail.jsp?id=568351

    World Small Animal Veterinary Association 2007 Vaccine Guidelines http://www.wsava.org/SAC.htm Scroll down to Vaccine Guidelines 2007 (PDF)

    The 2003 American Animal Hospital Association's Canine Vaccine Guidelines are accessible online at http://www.leerburg.com/special_report.htm .

    The 2006 American Animal Hospital Association's Canine Vaccine Guidelines are downloadable in PDF format at http://www.aahanet.org/PublicDocumen…s06Revised.pdf .

    Veterinarian, Dr. Robert Rogers,has an excellent presentation on veterinary vaccines at http://www.newvaccinationprotocols.com/

    October 1, 2002 DVM Newsletter article entitled, AVMA, AAHA to Release Vaccine Positions, http://www.dvmnewsmagazine.com/dvm/article/articleDetail.jsp?id=35171

    July 1, 2003 DVM Newsletter article entitled, What Do We Tell Our Clients?, Developing thorough plan to educate staff on changing vaccine protocols essential for maintaining solid relationships with clients and ensuring quality care http://www.dvmnewsmagazine.com/dvm/article/articleDetail.jsp?id=61696

    July 1, 2003, DVM Newsletter article, Developing Common Sense Strategies for Fiscal Responsibility: Using an interactive template to plan service protocol changes http://www.dvmnewsmagazine.com/dvm/article/articleDetail.jsp?id=61694

    Animal Wellness Magazine Article Vol. 8 Issue 6, How Often Does he REALLY Need A Rabies Shot Animal Wellness Magazine - devoted to natural health in animals

    The Rabies Challenge Animal Wise Radio Interview
    Listen to Animal Wise (scroll down to The Rabies Challenge 12/9/07)

    The Vaccine Challenge Animal Talk Naturally Online Radio Show ? The Vaccine Challenge - Show #91

    Rabies Shot Killed my Poodle May 28, 2008 Channel 5 News WCVB http://www.thebostonchannel.com:80/news/16410586/detail.html?rss=bos&taf=bos

    US Declared Canine-Rabies Free – CDC Announces at Inaugural World Rabies Day Symposium CDC Press Release - September 7, 2007

    Rabies Prevention – United States, 1991 Recommendations of the Immunization Practices Advisory Committee (ACIP), Center for Disease Control's Morbidity and Mortality Weekly March 22, 1991 / 40(RR03);1-19 http://www.cdc.gov/mmwr/preview/mmwrhtml/00041987.htm "A fully vaccinated dog or cat is unlikely to become infected with rabies, although rare cases have been reported (48). In a nationwide study of rabies among dogs and cats in 1988, only one dog and two cats that were vaccinated contracted rabies (49). All three of these animals had received only single doses of vaccine; no documented vaccine failures occurred among dogs or cats that had received two vaccinations. "

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